Viability of primary term cytotrophoblast cell culture in normoxia and hypoxia

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Viability of primary term cytotrophoblast cell culture in normoxia and hypoxia.

Cytotrophoblast (CT) cells isolated and purified from term placenta are able to differentiate into syncytiotrophoblast cells. Previous reports suggested that hypoxia is an inhibitor of this differentiation and also increases apoptosis. As visual observations of our CT cell cultures indicated a better development in hypoxia than in normoxia (defined as 2.5 and 21% O(2), respectively), we decided...

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Neurones to Normoxia and Hypoxia

1. Exposure of 10 R3-R13 neurones to a 115-min period of hypoxia resulted in depolarization of their membrane potentials (EM) from a mean of -46-9 ± 3-1 to-20-8 ± 4-4mV (S.E.). 2. Intracellular potassium ion activities (a^) decreased significantly from 118-9 ± 5 1 to 67-7 ± 8-5rnM-K. This is equivalent to a change in EKirom — 70-9 mV to —54-5 mV, which is insufficient to account for depolarizat...

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Hypoxia or in situ normoxia: The stem cell paradigm.

Although O(2) concentrations are considerably lowered in vivo, depending on the tissue and cell population in question (some cells need almost anoxic environment for their maintenance) the cell and tissue cultures are usually performed at atmospheric O(2) concentration (20-21%). As an instructive example, the relationship between stem cells and micro-environmental/culture oxygenation has been r...

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Introduction: Due to the changes that exercise brings to the body in hypoxic conditions, today hypoxic exercise is one of the most common types of exercise among most athletes, coaches and sports professionals to improve performance at sea level (1). In this regard, various types of hypoxia exercises, such as speed training in hypoxia, have recently been considered. Hypoxia exercises are usuall...

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ژورنال

عنوان ژورنال: Molecular Human Reproduction

سال: 2012

ISSN: 1360-9947,1460-2407

DOI: 10.1093/molehr/gas042